How do I make up a 50mM solution of spermine tetrahydrochloride?

I need to know how much spermine tetrahydrochloride and H2O to use to make up a 50mM solution. The molecular weight of spermine tetrahydrochloride is 348.2g and I would like to make up 20ml. Can you explain how to do this please?

Thanks.

50mM = .05M
20ml = .02l

.05M means you have .05 moles of spermine tetrahydrochloride in each liter of solution. You need .02l solution, so the moles of spermine tetrahydrochloride you need is:
.05M x .02l = .001 mol

The mass of spermine tetrahydrochloride you need:
.001 mol x 348.2g/mol = .3482g

So you need to add .3482g spermine tetrahydrochloride in to 20ml of water.

One Response to “How do I make up a 50mM solution of spermine tetrahydrochloride?”

  1. 50mM = .05M
    20ml = .02l

    .05M means you have .05 moles of spermine tetrahydrochloride in each liter of solution. You need .02l solution, so the moles of spermine tetrahydrochloride you need is:
    .05M x .02l = .001 mol

    The mass of spermine tetrahydrochloride you need:
    .001 mol x 348.2g/mol = .3482g

    So you need to add .3482g spermine tetrahydrochloride in to 20ml of water.
    References :

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